When does the London Eye River Cruise Operate? The London Eye River Cruise operates daily, with cruises departing at 45 minutes past the hour. Opening times may vary depending on seasonality, operational changes and special events, so please check opening times and operating hours. Buy Tickets Related articles Can I visit my secondary attractions first? What are your opening and closing hours? Can I visit all of my chosen attractions in one day? You close at 4pm, but my time slot is 3:45pm – do I only get 15 minutes inside? Where do I go to collect my tickets?