When does the London Eye River Cruise Operate? The London Eye River Cruise operates daily, with cruises departing at 45 minutes past the hour. Opening times may vary depending on seasonality, operational changes and special events, so please check opening times and operating hours. Buy Tickets Related articles What if I'm running late for my time slot? What are your opening and closing hours? When can I visit The London Dungeon? How much are tickets to Madame Tussauds London? When can I visit Shrek's Adventure London?